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24
2025-06
在传统中药宝库中,蟾酥以其强烈的药效闻名于世。蟾毒灵(Bufalin),正是从蟾酥中分离得到的一种关键活性物质,属于蟾蜍二烯内酯类化合物。它是蟾毒素(Bufotoxin)的水解产物之一,分子结构保留了这类物质的标志性特征:一个甾体核和一个位于C-17位的α-吡喃酮不饱和六元内酯环。这种独特的结构是其强大生物活性的基础。传统与现代之源:蟾酥与蟾毒灵蟾酥: 是中华大蟾蜍或黑眶蟾蜍等物种耳后腺及皮肤腺体的干燥分泌物,在传统医学(尤其是中医)中应用历史悠久,常用于治疗痈疽疔疮、咽喉肿痛、心力衰竭、疼痛及肿瘤等症。提取与存在: 蟾毒灵是蟾酥众多活性成分中的一种代表性单体化合物。现代药物化学技术(如溶剂提取、色谱分离)使我们能够从复杂的蟾酥混合物中纯化得到它,从而更精准地研究其特性和作用机制。令人瞩目的生物活性(药理作用)蟾毒灵最引人注目的是其广泛的、强效的生物活性,尤其在抗肿瘤领域研究最为深入:强效抗肿瘤作用(核心机制):抑制肿瘤细胞增殖。抑制肿瘤血管新生(抗血管生成)。影响端粒酶活性。调节自噬过程。抑制肿瘤细胞迁移和侵袭(抗转移)。激活Src激酶信号通路: 抑制钠钾泵会触发一系列细胞内信号级联反应,最终激活Src激酶。诱导多种癌细胞凋亡: 激活的Src可进一步调控下游如MAPK、PI3K/Akt、EGFR等信号通路,最终导致癌细胞周期阻滞(停在G2/M期)和程序性死亡(凋亡)。Na+/K+-ATP酶抑制: 这是蟾毒灵最经典的作用靶点。它通过高亲和力结合并抑制细胞膜上的Na+/K+-ATP酶(钠钾泵)。这不仅影响细胞的离子平衡和膜电位,更重要的是:其他抗肿瘤机制: 除上述核心机制外,研究还发现蟾毒灵能:研究热点: 蟾毒灵对多种癌细胞系(如肝癌、肺癌、胃癌、结直肠癌、白血病、胰腺癌、乳腺癌、前列腺癌等)在体外和部分动物模型中均显示出显著的抑制活性,常能克服某些化疗药物的耐药性,是抗肿瘤新药研发的重要候选分子。强心作用:作为蟾酥强心成分的代表,蟾毒灵具有典型的强心苷样作用。它通过抑制心肌细胞上的Na+/K+-ATP酶,导致细胞内钠离子浓度升高,继而通过Na+/Ca²⁺交换体使细胞内钙离子浓度升高,从而增强心肌收缩力(正性肌力作用)。但这同样也是其心脏毒性的基础。抗炎与镇痛作用:研究表明蟾毒灵能抑制多种炎症因子(如TNF-α, IL-1β, IL-6, IL-8, COX-2, iNOS)的产生和释放,减轻炎症反应。它也被证实具有一定的中枢性和外周性镇痛效果。抗病原体作用:对某些细菌、病毒(如乙型肝炎病毒)和寄生虫(如疟原虫)显示出一定的抑制活性。免疫调节作用:对免疫细胞功能具有复杂的调节作用,可能影响免疫应答。不容忽视的药理挑战:毒性蟾毒灵强大的生物活性是一把双刃剑,其显著的毒性是其临床应用的主要障碍:心脏毒性: 这是最严重的毒性。过量或不当使用会因过度抑制心肌Na+/K+-ATP酶,导致严重的室性心律失常(如室性心动过速、室颤)、房室传导阻滞,甚至心脏骤停。治疗窗(有效剂量与中毒剂量之间的范围)非常狭窄。胃肠道刺激: 可引起剧烈的恶心、呕吐、腹痛、腹泻等消化道症状。神经系统毒性: 可能导致头痛、头晕、抽搐、意识障碍等。局部刺激: 高浓度对皮肤和粘膜有强烈刺激性。现代研究致力于通过以下途径降低蟾毒灵毒性,提高安全性:结构修饰: 合成毒性更低、活性相当或更高的蟾毒灵衍生物或类似物。新型药物递送系统: 开发脂质体、纳米粒、靶向制剂等,提高肿瘤靶向性,减少对正常组织(尤其是心脏)的暴露。联合用药: 寻找与蟾毒灵协同增效并能降低其毒副作用的其他药物组合。应用现状与未来前景现状:蟾毒灵本身尚未作为单一成分药物被批准用于临床治疗(主要因其毒性问题)。含蟾毒灵的蟾酥或蟾酥制剂(如六神丸、华蟾素注射液/片)在严格遵循中医理论和规范的前提下,仍用于临床治疗某些肿瘤(常作为辅助用药)、炎症和疼痛。基础研究极其活跃,尤其在抗肿瘤机制探索和新型药物开发方面。前景与挑战:抗肿瘤新药研发: 克服心脏毒性是核心挑战。结构优化和靶向递送是极具前景的方向。寻找更特异的靶点或生物标志物以精准用药也至关重要。深入机制研究: 全面阐明其复杂的作用网络和毒性机制,为安全应用提供理论基础。临床转化: 需要设计严谨的临床试验来评估其修饰物或新制剂在人体中的有效性和安全性。结语蟾毒灵,这个源自古老蟾酥的天然化合物,以其强大的、多靶点的生物活性(尤其是卓越的抗癌潜力)持续吸引着科学家的目光。然而,其伴随而来的显著毒性,特别是致命的心脏风险,构成了其迈向现代临床应用的最大障碍。传统应用的智慧和现代科技的创新(结构修饰、靶向递送)正在合力破解这一难题。蟾毒灵的未来,很大程度上取决于我们能否成功驾驭其“双刃剑”特性,在保留其“利刃”(高效抗肿瘤活性)的同时,精心锻造出坚固的“剑鞘”(有效降低毒性),最终使其从一种剧毒的天然产物,蜕变为造福人类健康的精准武器。这条转化之路充满挑战,但每一步进展都可能为肿瘤治疗等领域带来新的希望。 -
23
2021-11
土贝母成分:土贝母皂苷甲,土贝母皂苷乙,土贝母皂苷丙,脱木糖土贝母苷丙 土贝母皂苷甲 98%克级 土贝母皂苷乙 98%克级土贝母皂苷丙 98%克级Tubeimoside I CAS号: 102040-03-9 分子式: C63H98O29 分子量: 1319.45g/mol 外 观:白色粉末 分 类: 萜类 来 源: 土贝母Tubeimoside II CAS号: 115810-12-3 分子式: C63H98O30 分子量: 1335.447g/mol 外 观:白色粉末 分 类: 萜类 来 源: 土贝母Tubeimoside Ⅲ CAS号: 115810-13-4 分子式: C64H100O31 分子量: 1365.473g/mol 外 观:白色粉末 分 类: 萜类 来 源: 土贝母土贝母成分:脱木糖土贝母苷丙 赶黄草成分:赶黄草苷A,赶黄草苷B 脱木糖土贝母苷丙 95%独家新品 赶黄草苷A 98%克级赶黄草苷B 98%Dexylosyltubeimoside Ⅲ CAS号: N/A 分子式: C59H92O27 分子量: 1233.36g/mol 外 观:白色粉末 分 类: 萜类 来 源: 土贝母Thonningianin A CAS号: 271579-11-4 分子式: C42H34O21 分子量: 874.713g/mol 外 观:淡黄色粉末 分 类: 酚类 来 源: 赶黄草Thonningianin B CAS号: 271579-12-5 分子式: C35H30O17 分子量: 722.608 g/mol 外 观:黄色粉末 分 类: 酚类 来 源: 赶黄草浙贝成分: 贝母辛,伊贝辛,贝母甲素,贝母乙素 贝母辛 98% 克级伊贝辛 98%新品贝母乙素 98% 克级Peimisine CAS号: 19773-24-1 分子式: C27H41NO3 分子量: 427.629g/mol 外 观:白色粉末 分 类: 生物碱 来 源: 浙贝Yibeissine CAS号: 143502-51-6 分子式: C27H41NO4 分子量: 443.63g/mol 外 观:白色粉末 分 类: 生物碱 来 源: 浙贝Peiminine CAS号: 18059-10-4 分子式: C27H43NO3 分子量: 429.645g/mol 外 观:白色粉末 分 类: 生物碱 来 源: 浙贝茯苓成分:茯苓酸,茯苓新酸A,茯苓新酸B,去氢茯苓酸,松苓新酸,猪苓酸C,去氢土莫酸,落新妇苷茯苓酸 98%克级新品 茯苓新酸A 98%新品茯苓新酸B 98%新品Pachymic acid CAS号: 29070-92-6 分子式: C33H52O5 分子量: 528.76g/mol 外 观:白色粉末 分 类: 萜类 来 源: 茯苓Poricoic acid A CAS号: 137551-38-3 分子式: C31H46O5 分子量: 498.704g/mol 外 观:白色粉末 分 类: 萜类 来 源: 茯苓Poricoic acid B CAS号: 137551-39-4 分子式: C30H44O5 分子量: 484.677g/mol 外 观:白色粉末 分 类: 萜类 来 源: 茯苓 去氢茯苓酸 98%新品松苓新酸 98%新品猪苓酸C 98%Dehydropachymic acid CAS号: 77012-31-8 分子式: C33H50O5 分子量: 526.747g/mol 外 观:白色粉末 分 类: 萜类 来 源: 茯苓Dehydrotrametenolic acid CAS号: 29220-16-4 分子式: C30H46O3 分子量: 454.695g/mol 外 观:白色粉末 分 类: 萜类 来 源: 茯苓Polyporenic acid C CAS号: 465-18-9 分子式: C31H46O4 分子量: 482.705g/mol 外 观:白色粉末 分 类: 萜类 来 源: 茯苓茯苓成分:去氢土莫酸 蟾酥稀有成分:蟾蜍五羟色胺,辛二酰精氨酸去氢土莫酸 98%克级 蟾蜍五羟色胺,5-羟色胺 98%克级辛二酰精氨酸 98%克级新品Dehydrotumulosic acid CAS号: 6754-16-1 分子式: C31H48O4 分子量: 484.721g/mol 外 观:白色粉末 分 类: 萜类 来 源: 茯苓5-hydroxytryptamine CAS号: 50-67-9 分子式: C10H12N2O 分子量: 176.219g/mol 外 观:白色粉末 分 类: 生物碱类 来 源: 蟾酥Suberylarginine CAS号: N/A 分子式: C14H26N4O5 分子量: 330g/mol 外 观:油状 分 类: 核苷类 来 源: 蟾酥蟾酥成分:沙蟾蜍精,3-乙酰基沙蟾毒精,伪异沙蟾毒精, 蟾毒灵,3-表蟾毒灵,3-羰基蟾毒灵,1β-羟基蟾毒灵,7β-羟基蟾毒灵,9-羟基蟾毒灵,19-羟基蟾毒灵, 蟾毒它灵,5β-羟基蟾毒它灵,华蟾毒它灵,去乙酰华蟾毒它灵,19-氧代华蟾毒它灵, 华蟾酥毒基,去乙酰华蟾毒精,3-表华蟾毒精,3-羰基华蟾毒精,华蟾毒精-3-丁二酰基-L-精氨酸脂, 和蟾毒它灵,3-表日蟾毒它灵, 酯蟾毒配基,3-表脂蟾毒配基,3-羰基脂蟾毒配基, 远华蟾蜍精,远华蟾毒精-3-戊二酰-L-精氨酸脂, 南美蟾毒精,惹斯蟾蜍精,蟾蜍它里定,嚏根草醇,蟾蜍特尼定,蟾蜍噻咛,Argentinogenin, 蟾蜍色胺,去氢蟾蜍色胺,华蟾蜍色胺,蟾蜍五羟色胺,N-甲基五羟色胺, 庚二酰精氨酸(Natural),次黄嘌呤(Natural),己二酰精氨酸(Natural),辛二酰精氨酸 3-乙酰基沙蟾毒精 98%克级新品去氢蟾蜍色胺 95%新品嚏根草配基,嚏根草苷元,蟾蜍它里定 98%Acetylarenobufagin CAS号: 184673-79-8 分子式: C26H34O7 分子量: 458.55g/mol 外 观:粉末 来 源:蟾酥 分 类:甾体化合物Dehydrobufo-tenine CAS号: 17232-69-8 分子式: C12H15N2O+ 分子量: 203.265g/mol 外 观:白色粉末 来 源:蟾酥 分 类:甾体化合物Hellebrigenin CAS号: 465-90-7 分子式: C24H32O6 分子量: 416.514g/mol 外 观:白色粉末 来 源:蟾酥 分 类:甾体化合物 -
23
2021-11
猫须草成分: 半齿泽兰素,半齿泽兰素-5甲醚,5,6-二羟基-7,4'-二甲氧基黄酮 ,甜橙黄酮半齿泽兰素 98%克级新品半齿泽兰素-5甲醚 98%新品 5,6-二羟基-7,4'-二甲氧基黄酮 98%克级Eupatorin CAS号: 855-96-9 分子式: C18H16O7 分子量: 344g/mol 外 观:黄色粉末 分 类:黄酮类 来 源:猫须草Eupatorin-5-methyl ether CAS号: 21764-09-0 分子式: C19H18O7 分子量: 358.11g/mol 外 观:黄色粉末 分 类:黄酮类 来 源:猫须草Ladanein CAS号: 10176-71-3 分子式: C17H14O6 分子量: 314g/mol 外 观:黄色粉末 分 类:黄酮类 来 源:猫须草 猫须草成分: 甜橙黄酮 茶稀有成分:香叶醇-樱草糖苷,芳樟醇-樱草糖苷甜橙黄酮 98%新品香叶醇-樱草糖苷 98%新品 芳樟醇-樱草糖苷 98%克级Sinensitin CAS号: 2306-27-6 分子式: C20H20O7 分子量: 372.37g/mol 外 观:黄色粉末 分 类:黄酮类 来 源:猫须草Geraniol-primeveroside CAS号: N/A 分子式: C21H36O10 分子量: 448g/mol 外 观:粉末 分 类:糖苷类 来 源:茶Linalool-primeveroside CAS号: 10176-71-3 分子式: C21H36O10 分子量: 448.51g/mol 外 观:粉末 分 类:糖苷类 来 源:茶地黄成分: 地黄苦苷,梓醇,密力特苷,地黄苷D,尿苷(natural) 地黄苦苷 98%独家新品 梓醇 98%克级 密力特苷 98%Rehmapicroside CAS号: 104056-82-8 分子式: C16H26O8 分子量: 346.376g/mol 外 观:淡黄色粉末 分 类: 糖苷类 来 源: 地黄Catalpol CAS号: 2415-24-9 分子式: C15H22O10 分子量: 362.331g/mol 外 观:白色粉末 分 类: 萜类 来 源: 地黄Melittoside CAS号: 19467-03-9 分子式: C21H32O15 分子量: 524.472g/mol 外 观:淡黄色粉末 分 类: 萜类 来 源: 地黄地黄成分: 地黄苷D,尿苷(natural) 橄榄成分:Oleuroside 地黄苷D 95%克级新品 尿苷(natural) 98%克级 Oleuroside 98%克级新品Rehmannioside D CAS号: 81720-08-3 分子式: C27H42O20 分子量: 686.613g/mol 外 观:淡黄色粉末 分 类: 糖苷类 来 源: 地黄Uridine CAS号: 58-96-8 分子式: C9H12N2O6 分子量: 244.2g/mol 外 观:粉末 分 类: 核苷类 来 源: 地黄Oleuroside CAS号: 116383-31-4 分子式: C25H32O13 分子量: 540.52g/mol 外 观:粉末 分 类: 萜类 来 源: 橄榄 -
23
2021-11
王不留行成分:王不留行环肽A,王不留行环肽B,王不留行环肽C,王不留行黄酮苷, 王不留行黄酮苷E,异牡荆素-2''-O-阿拉伯糖苷,刺桐碱 王不留行环肽A 98%克级新品王不留行环肽B 98%新品王不留行环肽C 98%新品Segetalin A CAS号: 161875-97-4 分子式: C31H43N7O6 分子量: 609.7g/mol 外 观:粉末 来 源:王不留行 分 类:氨基酸/肽类Segetalin B CAS号: 164991-89-3 分子式: C24H32N6O5 分子量: 484.557g/mol 外 观:粉末 来 源:王不留行 分 类:氨基酸/肽类Segetalin C CAS号: 177602-12-9 分子式: C43H56N8O8 分子量: 812.97g/mol 外 观:粉末 来 源:王不留行 分 类:氨基酸/肽类王不留行黄酮苷 98%克级新品王不留行黄酮苷E 98%新品异牡荆素-2''-O-阿拉伯糖苷 98%新品Vaccarin CAS号: 53452-16-7 分子式: C32H38O19 分子量: 726.637g/mol 外 观:粉末 来 源:王不留行 分 类:黄酮类Vaccarin E CAS号: 2252345-81-4 分子式: C42H48O22 分子量: 904.82g/mol 外 观:粉末 来 源:王不留行 分 类:黄酮类Isovitexin 2''-O-arabinoside CAS号: 53382-71-1 分子式: C26H28O14 分子量: 564.5g/mol 外 观:粉末 来 源:王不留行 分 类:黄酮类王不留行成分:刺桐碱 金线莲成分: 金线莲苷,Goodyeroside A刺桐碱 98%克级新品金线莲苷 98%克级新品Goodyeroside A 98%克级新品Hypaphorine CAS号: 487-58-1 分子式: C14H18N2O2 分子量: 246.31g/mol 外 观:粉末 来 源:王不留行 分 类:生物碱类Kinsenoside CAS号: 151870-74-5 分子式: C10H16O8 分子量: 264.23g/mol 外 观:粉末 来 源:金线莲 分 类:糖苷类Goodyeroside A(Kinsenoside的差向异构体) CAS号: N/A 分子式: C10H16O8 分子量: 264g/mol 外 观:粉末 来 源:金线莲 分 类:糖苷类 -
13
2021-10
沙葱成分:槲皮素3,7(葡醛酸糖)-三糖;槲皮素3,7-二葡萄糖,4’醛酸糖;山奈酚3,7二葡萄糖苷;山奈酚3,7-二葡萄糖,4’醛酸糖;山奈酚3(1-4),7-三葡萄糖,4’醛酸糖;1-咖啡酸葡萄糖(1-4葡萄糖);1,2咖啡酸基葡萄糖,存在α β两种糖构型;单葡萄糖3位连接咖啡酸羧基;L-氨基酸;N-对香豆酰酪胺;N-反式-阿魏酰基酪胺;N-反式-阿魏酰-3-甲氧基酪胺;山柰酚-3-O-芸香糖苷
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Conditions for Making a Reference Standard Return
The product(s) must be unopened and in its original packaging.
The product(s) must be stored appropriately, as indicated on the certificate of analysis.
In the case the return is being requested due to an error on Herbest’s behalf, we will investigate the root cause of the error. If it is deemed a Herbest error, the product(s) can be returned regardless if it meets the conditions above or not.
Restocking and Shipping Fees
All returns are subject to a 20% restocking fee (unless deemed a Herbest error after investigation).
Shipping fees will not be refunded unless the cause for the return was found to be an error on Herbest’s part.
Return Instructions
1. In order to initiate a return, please contact your Herbest representative and provide the following information:
The invoice document number for purchase
The lot number(s) for the product(s) intended to be returned
Reason for return of the product(s)
2. After receiving the information listed above, your herbest representative will generate a return number for you to authorize the return.
3. Once the return number is received, please ship the product(s) with the return number (RTN) referenced on the package. Ensure the product(s) is/are appropriately packaged and stored (i.e. -20oC stored items need to be shipped overnight with an icepack, -80oC items with dry ice). The shipment will be made out to the following address:
Baoji Herbest Bio-Tech Co.,Ltd
Attn:[herbest representative]
BaoTao Road Baoji City Shannxi provience China (zip: 721013)
Tel:+86-917-8888998
4. Once the product(s) is/are received and inspected, your representative will issue a return (RTN) document receipt. This document will reflect the funds credited back to you for return of the product(s), minus any applicable restocking or shipping fees. Purchases made via prepayment or NET 30 will have a credit memo for the balance added to their account.
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Herbest supplies a wide range of phytochemical reference standards to highly specialized and very rare phytochemicals available in extremely small quantities in nature. Our specialtis making all of these products available to the industry with extensive testing and characterization data on each. Herbest committed to providing our customers with cost effective, high quality reference tandards. We strive to provide that influence how long it will take to fill any individual order and we hope this information will assist you in understanding our production process and lead time estimates.
Production Phase 1 :Starting Materials
The first phase in our production process is to acquire the necessary starting materials. In the case that the compound will be isolated directly from plant material, we must first acquire the relevant biomass. Availability of suitable biomass can vary widely and might require waiting for the next harvest time to maximize the level of the target phytochemical. Growing and harvest conditions can dramatically affect the phytochemical content and in extreme cases, normally prominent target compounds might be missing entirely from a particular harvest. This certainly can complicate the production process. All biomass materials have to be verified for compliance with Herbest quality requirements. Compounds
produced synthetically also require properly qualified starting materials. In this case, the commercial availability of the starting materials directly affects our ability to begin the production process.
Production Phase 2: Production of Target Compound
Once all the starting materials are received and qualified, we can begin with the production of the target compound. Depending on production prioritization, some materials may have to wait in the production queue before work begins. Both isolation and synthesis productions are typically multistep processes and can take from just a few days to several weeks to complete the whole process. Usually production steps proceed smoothly but sometimes unanticipated technical challenges can arise.
Production Phase 3: Quality Testing and Verification
In this phase, the isolated or synthesized materials are tested and verified. The results must meet the final identity, purity, and quality requirements for the desired product. The data package from this characterization testing is then used to generate the product certificate of analysis (C of A) appropriate to the product grade. The C of A then undergoes a formal review by the Quality Assurance group that operates independently of the chemists performing the tests and preparing the documentation. Oncethe C of A is completed, the material can then go into stock and orders are filled. Usually quality testing and verification is a smooth process but in some cases, material that was promising during Phase 2, will fail final requirements during Phase 3 testing in which case, the process must start over.
Herbest takes pride in providing its customers with the highest quality natural product reference standards available to the industry.Please contact your Technical Sales Representative with any additional questions you may have.
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Herbest reference standards are supplied in the powder, crystal, liquid, or oily form. The reference standards may also be supplied in various containment forms including screw top vials, crimp top vials, or sealed ampules (depending on the best form of enclosure determined by Herbest). This can present a recovery challenge when the material clings to the insides of the sample bottle/vial. Proper handling is especially important with small amounts where a greater percentage of the sample may be clinging to the sides of the bottle, screw cap or sto per. Please note that Herbest does not recommend scraping the vial because sample recovery will be quite low. The following procedure for sample handling will allow for the greatest recoveries and proper dilution. Please note: Oily or liquid standards can be difficult to see and the bottle may appear empty. Examples of such oily standards are: Gingerol, Pinane, Shogaol,Rosin and Bakuchiol.
Proper Standard Material Recovery and Dilution Procedure:
1. Use a 5 decimal place (0.00001g) analytical balance for weighing.
2. Weigh the full sample bottle and vial cap.
3. Record the exact weight of the full bottle and cap/stopper, in your laboratory notebook.
4. Remove the screw top of the vial and add about 1mL of the appropriate solvent to the sample bottle and immediately replace the cap/stopper. If the bottle/vial has a crimp top, draw 1ml of the appropriate solvent into a clean syringe (with a rigid needle tip) and pierce the crimp top septum and expel the 1ml of solvent into the vial.
5. Shake the bottle/vial multiple times over 5 minutes and allow the standard to completely dissolve.
6. Open the screw/crimp top vial and pipette or pour the dissolved standard from the bottle into a volumetric flask (Crimp top vial will need a crimp top remover tool).
7. Use an appropriately sized volumetric flask for the sample dilution.
8. Rinse the bottle/vial and cap/stopper each into the volumetric flask three times.
9. Completely dry the empty bottle and cap/stopper under nitrogen flow.
10. Weigh the, now empty, dried bottle and cap/stopper and record the weight in your laboratory notebook.
11. Partially fill the volumetric flask with the chosen solvent and mix thoroughly.
12. Then dilute to the mark and mix again by inverting several times.
13. Calculate the total weight of standard removed from the bottle/vial by subtracting the dry weight from the original weight.
14. Calculate the concentration of the diluted standard by dividing the calculated standard weight (from step above) by the volume of the volumetric flask.
15. Consider portioning off your diluted standard into smaller bottles for later use and to prevent contamination of your standard solution.
16. Ideally, store the reference standard solution in a cool, dry place out of light per recommended storage conditions found on the Certificate of Analysis and/or label.
please contact Herbest with any further questions about proper sample handling specific to your use.
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Herbest is pleased to offer a wide range of reference standards in our phytochemical catalog. All materials listed are supplied either neat or in solution and sold by either a weight or volume quantity. Sometimes our clients have questions about how much product is in each bottle or ampoule. This FAQ should help to address such questions.
Herbest overfills each unit of product by up to 0.2 mg. This typically means that a 5mg unit may contain between 5.0 and 5.2mg of material; a 10mg unit contains between 10.0 and 10.2mg of material. Products sold by volume are overfilled by 0.1ml.
The use of accurate weight measurements and fully characterized Primary grade reference standards is crucial for proper quantitative analysis. A five place balance (measuring accurately to 0.00005mg) is required for use in quantitative work.
Herbest recommends that a “weigh by difference” technique be used when preparing your reference standard solutions for use in the laboratory. Especially when dealing with oily, sticky, fluffy, or static prone compounds, improper handling can result in large losses of material between the original packaging and the prepared solution in your laboratory.Because of potential material loss in transferring by the end user, Herbest does not supply an exact weight value for each unit of reference standard. Even if an exact weight was supplied, the end user must accurately weigh the amount of product that goes into their standard preparation as this minimizes analytical error.
For special projects, Herbest can supply exact weights per vial when requested by our clients. An additional fee may apply in such cases. Please contact your herbest sales representative for more information if this service would be useful for you.
Herbest packages all items carefully to minimize any product loss. However, sometimes shipping conditions beyond our control can cause leakage or an otherwise reduced quantity of material. If you suspect that your order contains less material than it should, please first follow the weighing protocol as detailed in Herbest Recovery and Dilution Procedures. If after using this protocol, you cannot verify the correct quantity, please contact your Herbest sales representative with the weight measurements and any photos showing bottle leakage that might help us in trouble shooting the issue.